Developments in 3D microscopy
نویسندگان
چکیده
منابع مشابه
Modern Developments in Electron Microscopy
The caliber of many of the papers was less than optimum. For instance, in the paper on, "The effects of Insulin on Water Intake in the Rat," the author made no attempt to determine if hypoglycemia, shock, or anoxia had occurred after 16-32 units of crystalline insulin was given intravenously to rats. For the reader interested in the complex physio-psychological problem of thirst this symposium ...
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Cryo-electron microscopy and single-particle 3D image reconstruction techniques have been used to examine a broad spectrum of samples ranging from 500 kDa protein complexes to large subcellular organelles. The attainable resolution has improved rapidly over the past few years. Structures of both symmetric and asymmetric assemblies at approximately 7.5 A have been reported. Together with X-ray c...
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Location Based Services (LBS) are gaining much importance for all kind of human activities ranging from tourist navigation to support of rescue teams in disaster management. LBS have been developed as an independent from GIS stream for wireless services, but nowadays about 80% of all supplied data is spatially related, i.e. they are presented in a form of 2D map (raster of vector). LBS is becom...
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We present a new optimization concept for 3D multiphoton fluorescence microscopy by finding the optimal excitation beam giving rise to the smallest possible light-emitting volume or the highest possible signal to noise ratio (SNR).
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In previous studies, we examined three-dimensiional images of a latex bead containing a surface layer of fluorescence whose thickness was determined by physical sectioning. Confocal images of the bead from six different microscopes all exhibited a significantly thicker fluorescent shell than actually present. In contrast, deconvolved wide-field images of the bead produced an image with the corr...
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ژورنال
عنوان ژورنال: SPIE Newsroom
سال: 2007
ISSN: 1818-2259
DOI: 10.1117/2.1200705.0707